Our standard transcriptome sequencing protocol is conducted using non-stranded Poly-A selection for mRNA. This protocol is ideal for researchers interested in expression fold changes, pathway analysis, and identification of known variants in eukaryotic organisms when samples are derived from fresh, non-blood tissue or cells.*
*We offer a wide of array of “non-standard” RNA Sequencing services. Contact Us to discuss pricing and details.
Standard Protocol: Non-Stranded Poly-A Selection
Standard Coverage: 20M Read Pairs (PE150)
Turnaround: Two Weeks
Standard Deliverable: Demultiplexed FASTQ File(s) by FTP
Material Submitted: Total RNA**
Amount: 200 nanograms
Purity: RIN ≥ 6
Volume: 20mL
Sample requirements are suggestions based on our experience and are the best indicators of a successful sequencing project. Samples with less than optimal quality may still proceed based on need and overall quality scores or an alternative kit/protocol may be suggested.
**Extraction can be added based on source material and will incur an additional charge.
Our standard transcriptome sequencing protocol is conducted using non-stranded Poly-A selection for mRNA. This protocol is ideal for researchers interested in expression fold changes, pathway analysis, and identification of known variants in eukaryotic organisms when samples are derived from fresh, non-blood tissue or cells.*
*We offer a wide of array of “non-standard” RNA Sequencing services. Contact Us to discuss pricing and details.
Standard Protocol:
Standard Coverage:
Turnaround:
Standard Deliverable: Demultiplexed FASTQ File(s) by FTP
Material Submitted:
Amount:
Purity:
Volume: 20mL
Sample requirements are suggestions based on our experience and are the best indicators of a successful sequencing project. Samples with less than optimal quality may still proceed based on need and overall quality scores or an alternative kit/protocol may be suggested.
**Extraction can be added based on source material and will incur an additional charge.
Our standard transcriptome sequencing protocol is conducted using non-stranded Poly-A selection for mRNA. This protocol is ideal for researchers interested in expression fold changes, pathway analysis, and identification of known variants in eukaryotic organisms when samples are derived from fresh, non-blood tissue or cells.*
*We offer a wide of array of “non-standard” RNA Sequencing services. Contact Us to discuss pricing and details.
Standard Protocol:
Standard Coverage:
Turnaround:
Standard Deliverable: Demultiplexed FASTQ File(s) by FTP
Material Submitted:
Amount:
Purity:
Volume: 20mL
Sample requirements are suggestions based on our experience and are the best indicators of a successful sequencing project. Samples with less than optimal quality may still proceed based on need and overall quality scores or an alternative kit/protocol may be suggested.
**Extraction can be added based on source material and will incur an additional charge.
